candida albicans strain sc5314  (ATCC)

Journal: RSC Chemical Biology

Article Title: Prochelators modulate azole activity against Candida albicans in a metal-dependent manner

doi: 10.1039/d6cb00073h

Figure Lengend Snippet: (A) Effect of prochelators (AH1-S) 2 , (IT1-S) 2 , (244mTC-S) 2 , and (TC1-S) 2 on growth of C. albicans strain SC5314. (AH1-S) 2 and (IT1-S) 2 decrease growth at 24 h while (244mTC-S) 2 and (TC1-S) 2 are inactive at the concentrations tested. At 48 h, cells have recovered growth. (B) (AH1-S) 2 and (IT1-S) 2 decrease growth at 24 h of C. albicans Y172, a fluconazole-resistant clinical isolate. Cell growth in SD medium was measured by OD 600 . ( n = 2–5 biological replicates each of n = 1–3 technical replicates). Error bars represent Standard Error of Mean (SEM). Welch ANOVA p -value < 0.0001, Dunnett's T3 multiple comparison p -value = 0.032 (*), 0.0021 (**) or < 0.0001 (****).

Article Snippet: Candida albicans laboratory strain SC5314 (MYA 28776), purchased from ATCC, was used for all experiments unless otherwise noted .

Techniques: Comparison

Journal: RSC Chemical Biology

Article Title: Prochelators modulate azole activity against Candida albicans in a metal-dependent manner

doi: 10.1039/d6cb00073h

Figure Lengend Snippet: Left column (A) (AH1-S) 2 and right column (B) (IT1-S) 2 modulate growth inhibitory effect of azoles against C. albicans SC5314 (SD medium, 48 h, n = 2–4 biological replicates of n = 1–3 technical replicates). Experiments with (AH1-S) 2 were performed with at least one biological replicate under minimal light exposure.

Article Snippet: Candida albicans laboratory strain SC5314 (MYA 28776), purchased from ATCC, was used for all experiments unless otherwise noted .

Techniques:

Journal: RSC Chemical Biology

Article Title: Prochelators modulate azole activity against Candida albicans in a metal-dependent manner

doi: 10.1039/d6cb00073h

Figure Lengend Snippet: (A)–(D) Metals were added into SD medium made without the designated metal to give a concentration that ranged from deficient to excess. Under these conditions, C. albicans SC5314 were not treated (NT, black bars, circles) or treated with 8 µM fluconazole (purple bars, squares), 15 µM (AH1-S) 2 (pink bars, triangles), (IT1-S) 2 (light blue, triangles), or combined 15 µM prochelator plus 8 µM fluconazole (green triangles or dark blue rhombus). Growth was measured by OD 600 after 48 h and normalized relative to non-treated cells. (E) Cidality assays on YPD agar plates after 48 h. Non-toxic levels of copper convert (AH1-S) 2 from fungistatic to fungicidal. The images above are representative of at least n = 2 biological replicates of n = 1–3 technical replicates. Error bars represent SEM.

Article Snippet: Candida albicans laboratory strain SC5314 (MYA 28776), purchased from ATCC, was used for all experiments unless otherwise noted .

Techniques: Concentration Assay

Journal: RSC Chemical Biology

Article Title: Prochelators modulate azole activity against Candida albicans in a metal-dependent manner

doi: 10.1039/d6cb00073h

Figure Lengend Snippet: Levels of (A) iron, (B) manganese, (C) zinc, and (D) copper in C. albicans SC5314 after 90 min and 6 h of treatment with 15 µM (AH1-S) 2 , 10 µM fluconazole, 10 µM copper, or a combination. Data are plotted as fold change relative to the respective metal concentration for non-treated cells (represented by dashed line). Error bars represent SEM; n = 2–3 biological replicates with multiple technical replicates; at least one biological replicate performed under minimal light conditions. Welch's ANOVA p -value = 0.0001, Dunnett's T3 p -value = 0.0438 (*), 0.0075 (**), and 0.0001 (***).

Article Snippet: Candida albicans laboratory strain SC5314 (MYA 28776), purchased from ATCC, was used for all experiments unless otherwise noted .

Techniques: Concentration Assay

Journal: RSC Chemical Biology

Article Title: Prochelators modulate azole activity against Candida albicans in a metal-dependent manner

doi: 10.1039/d6cb00073h

Figure Lengend Snippet: (A) Percent colony forming units (CFUs)/mL of C. albicans SC5314 co-cultured with macrophages relative to the CFU/mL of the non-treated mono-culture. (B) Macrophage cytotoxicity as measured by LDH assay. (C) Images of cells in 96-well plates under co-culture conditions. Left to right: NT, 1.5 µM (AH1-S) 2 , 6.5 µM (AH1-S) 2 , 25 µM (AH1-S) 2 , 2 µM fluconazole, and 2 µM fluconazole + 6.5 µM (AH1-S) 2 . Cells were grown in DMEM under mammalian cell culture conditions. Red arrow – macrophage; blue arrow – C. albicans hyphae.

Article Snippet: Candida albicans laboratory strain SC5314 (MYA 28776), purchased from ATCC, was used for all experiments unless otherwise noted .

Techniques: Cell Culture, Lactate Dehydrogenase Assay, Co-Culture Assay